논문 국내 국내일반학술지 Ellman 효소법에 의한 대전시 상수도내 살충제의 잔류농도 결정 및 그 대책에 관한 연구
- 학술지 구분 국내일반학술지
- 게재년월 1999-02
- 저자명 이봉호공동(교신)
- 학술지명 한국환경과학회지
- 발행처명 한국환경과학회
- 발행국가 국내
- 논문언어 한국어
홈페이지논문 초록 (Abstract)
The degree of pesticides accumulation in tap water in Taejon from June 1995 to Apr 1996 was measured by Ellman's coupled enzyme assay. Since organic phosphate and carbamate pesticides specifically inhibit the neurotransmitter modulating enzyme acetylcholinesterase(AChE), the enzyme activity can be used as a diagnosis for the pesticides accumulation in water and various samples. During the period of this study, the enzyme activity was changed almost every week. The lowest enzyme activity was 64 % of that of the control reaction and there are several days showing about 100 % enzyme activity. In general, the enzyme activity is higher in summer than other seasons especially early spring times. The pH value of tap water was very close to neutral(pH 7.0) and it seems that the enzyme activity was not affected by the small pH changes. Either boiling of tap water or addition of NaOH solution decomposed the pesticide components. These results show that AChE assay is a convenient, sensitive, and reliable method for detection of pesticides in water samples.