논문 국내 국제전문학술지(SCI급) Antioxidant and Anti-inflammatory Activities of the Halophyte Cyrtomium falcatum
- 학술지 구분 국제전문학술지(SCI급)
- 게재년월 2021-09
- 저자명 이봉호공동(참여),서영완,김호준,심현보,공창숙,김현모
- 학술지명 Ocean and Polar Research
- 발행처명 Korea Ocean Research and Development Institute
- 발행국가 국내
- 논문언어 한국어
홈페이지논문 초록 (Abstract)
In the present study, the halophyte C. falcatum extract and its solvent fractions (n-hexane, 85% aqueous methanol, n-butanol, and water) were evaluated for antioxidant and anti-inflammatory activities. Antioxidative ability was measured by DPPH radical, intracellular reactive oxygen species (ROS) and peroxynitrite scavenging, DNA oxidation inhibition, and ferric reducing antioxidant power (FRAP). For DPPH radical and peroxynitrite scavenging, DNA oxidation inhibition, and FRAP, 85% aq.MeOH and n-BuOH fractions showed significant scavenging activity. For production of intracellular ROS in HT-1080 cells, 85% aq.MeOH fraction showed the highest scavenging activity. In addition, anti-inflammatory activity was also assessed by measuring the inhibitory effect against mRNA expression of pro-inflammatory factors (NO, IL-1β, IL-6 and COX-2) in LPS-stimulated Raw 264.7 macrophages. For NO production, crude extract exhibited a strong inhibitory effect at a concentration of 100 μg/ml. For mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6, and COX-2), n-BuOH greatly suppressed expression levels of IL-1β and IL-6 at 100 μg/ml concentration while 85% aq. MeOH fraction significantly inhibited that of COX-2 even at 100 μg/ml. These results suggest that C. falcatum may be used as a potential source for the development of a natural antioxidant or anti-inflammatory agent.