논문 국내 국제전문학술지(SCI급) Water Extract of Mixed Mushroom Mycelia Grown on a Solid Barley Medium Is Protective against Experimental Focal Cerebral Ischemia
- 학술지 구분 국제전문학술지(SCI급)
- 게재년월 2021-06
- 저자명 이봉호공동(참여),Seung Yun Han,Yung Choon Yoo,Do Kyung Kim,Young Gil Jeong,Nam Seob Lee,Ki Duck Kim,Ah La Choi,Ji Hyun Moon,Chan Eui Song,Hyun Young Park,Jong Yea Park,Mi Na Park,Shin Hye Kim,Ji Heun Jeong
- 학술지명 CURRENT ISSUES IN MOLECULAR BIOLOGY
- 발행처명 CAISTER ACAD PRESS
- 발행국가 국내
- 논문언어 한국어
홈페이지논문 초록 (Abstract)
Although the individual consumption of medicinal mushrooms, including Phellinus linteus (PL), Ganoderma lucidum (GL), and Inonotus obliquus (IO), is known to be neuroprotective, the associated mechanisms underlying their therapeutic synergism on focal cerebral ischemia (fCI) have yet to be elucidated. This study aimed to demonstrate the neuroprotective effects of mixed mushroom mycelia (MMM) against experimental fCI. The water-fractions, ethanolic-fractions, and ethyl acetate-fractions of the MMM (PL, GL, and IO) grown in a barley medium using solid-state fermentation techniques were prepared and their protective effects against glutamate-induced excitotoxicity were compared in PC-12 cells. After the identification of the water extracts of MMM (wMMM) as the most suitable form, which possessed the lowest toxicity and highest efficacy, further analyses for evaluating the anti-apoptotic effects of wMMM, including Hoechst 33258-based nuclear staining, fluorescence-activated cell sorting, and reactive oxygen species (ROS) detection assays, were performed. Rats were subjected to a 90 min middle cerebral artery occlusion and reperfusion, after which a wMMM treatment resulted in significant dose-dependent improvements across a number of parameters. Furthermore, measurements of intracellular ROS and levels of antioxidant enzymes revealed a wMMM-mediated ROS attenuation and antioxidant enzyme upregulation. We suggest that wMMM is neuroprotective against fCI through its anti-apoptotic and anti-oxidative effects.