연구홍보포털

논문 초록 (Abstract)

The hallmarks of diabetics are insufficient secretion of insulin and dysregulation of glucagon. It is critical to understand release mechanisms of insulin, glucagon, and other hormones from the is lets of Langerhans. In spite of remarkable advancements in diabetes res earch and practice, robust and reproducible models that can measure pancreatic -cell functionare lacking. Here, a microphysiolog ical analysis platform (MAP) that allows the uniform 3D spheroid f ormation of pancreatic -cell islets, large-scale morphological phenoty ping, and gene expression mapping of chronic glycemia and lipidemia dev elopment is reported. The MAP enables the scaffold-free formation of d ensely packed -cell spheroids (i.e., multiple array of 110bioreacto rs) surrounded with a perfusion flow network inspired by physiolo gically relevant microenvironment. The MAP permits dynamic perturbati ons on the -cell spheroids and the precise controls of glycemia and lipi demia, which allow us to confirm that cellular apoptosis in the -cel l spheroid under hyperglycemia and hyperlipidemia is mostly dependent to a reactive oxygen species-induced caspase-mediated pathway. The -cells' MAP might provide a potential new map in the pathophysiologic al mechanisms of cells.