논문 해외 국제전문학술지(SCI급) Resolution enhancement in continuous-wave stimulated emission depletion microscopy by excitation beam with modulated vortex-phased azimuthally polarized light
- 학술지 구분 국제전문학술지(SCI급)
- 게재년월 2020-11
- 저자명 김완진,임건,박노철
- 학술지명 OPTICS AND LASERS IN ENGINEERING
- 발행처명 ELSEVIER SCI LTD
- 발행국가 해외
- 논문언어 외국어
- 전체저자수 3
홈페이지논문 초록 (Abstract)
In this paper, we report an optical system that can effectively enhance the resolution of continuous-wave (CW) stimulated emission depletion (STED) microscopy by applying an amplitude-modulated azimuthally polarized light with vortex phase (AV) as an excitation beam. Based on the vector field diffraction theory, we analyzed electric fields near the focal plane for various polarization states and rotationally symmetric amplitude modulation conditions. From the analysis, it was found that by applying the amplitude modulated AV beam by the ring-shaped blocking aperture, the resolution in the lateral direction can be improved while minimizing the degradation of the resolution in the axial direction. The reported novel excitation method was experimentally verified based on the imaging of gold beads and fluorescent nano-beads. Accordingly, it was confirmed that the lateral resolution was improved by similar to 20% and the spot elongation in the axial direction was effectively suppressed.